Review



p stat3 s727 9134t  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology p stat3 s727 9134t
    P Stat3 S727 9134t, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 4469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/Stat3/bio_rxiv__2025__11__17__688926-132-42-55
    Average 96 stars, based on 4469 article reviews
    p stat3 s727 9134t - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Saline:

    Article Title: PTEN ameliorates autoimmune arthritis through down-regulating STAT3 activation with reciprocal balance of Th17 and Tregs.
    Article Snippet: Proteins were loaded onto 10% polyacrylamide gels and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by transferring to nitrocellulose membranes (Invitrogen Life Technologies, Carlsbad, CA, USA). .. Membranes were blocked with 5% (w/v) non-fat milk in Tris-buffered saline containing 0.1% Tween-20 for 1 h followed by incubation with antibodies against p-STAT3 Y705, p-STAT3 S727, t-STAT3 (Cell signaling), and β -actin (Santa Cruz Biotechnology) overnight at 4 °C. .. Immunoreactivity was determined using enhanced chemiluminescence reagents (Amersham Biosciences, Piscataway, NJ, USA).

    Incubation:

    Article Title: PTEN ameliorates autoimmune arthritis through down-regulating STAT3 activation with reciprocal balance of Th17 and Tregs.
    Article Snippet: Proteins were loaded onto 10% polyacrylamide gels and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by transferring to nitrocellulose membranes (Invitrogen Life Technologies, Carlsbad, CA, USA). .. Membranes were blocked with 5% (w/v) non-fat milk in Tris-buffered saline containing 0.1% Tween-20 for 1 h followed by incubation with antibodies against p-STAT3 Y705, p-STAT3 S727, t-STAT3 (Cell signaling), and β -actin (Santa Cruz Biotechnology) overnight at 4 °C. .. Immunoreactivity was determined using enhanced chemiluminescence reagents (Amersham Biosciences, Piscataway, NJ, USA).

    other:

    Article Title: CXCR4 uses STAT3-mediated slug expression to maintain radioresistance of non-small cell lung cancer cells: emerges as a potential prognostic biomarker for lung cancer
    Article Snippet: Antibody such as p-STAT3 (S727) and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). p-STAT3 (Y705) and Slug were obtained from Cell Signaling Biotechnology (Denvers, MA).

    Bioprocessing:

    Article Title: MSC-exosomes pretreated by Danshensu extracts pretreating to target the hsa-miR-27a-5p and STAT3-SHANK2 to enhanced antifibrotic therapy
    Article Snippet: .. E-cadherin, VEGF-A (BS6496), STAT3 (BS1336), p-STAT3 (S727) (AP0248), p-STAT3 (T705) (AP0247), CTGF, FN, and HIF-1A rabbit monoclonal antibodies were purchased from Santa Cruz Biotechnology. .. High-sensitivity chemiluminescent detection solution was obtained from Amersham Life Sciences (Buckinghamshire, UK).

    Article Title: MSC-exosomes pretreated by Danshensu extracts pretreating to target the hsa-miR-27a-5p and STAT3-SHANK2 to enhanced antifibrotic therapy.
    Article Snippet: .. E-cadherin, VEGF-A (BS6496), STAT3 (BS1336), p-STAT3 (S727) (AP0248), p-STAT3 (T705) (AP0247), CTGF, FN, and HIF-1A rabbit monoclonal antibodies were purchased from Santa Cruz Biotechnology. .. High-sensitivity chemiluminescent detection solution was obtained from Amersham Life Sciences (Buckinghamshire, UK).

    Molecular Weight:

    Article Title: Molecular mechanism of Danshenol C in reversing peritoneal fibrosis: novel network pharmacological analysis and biological validation.
    Article Snippet: .. Human peritoneal mesothelial cell line (HMrSV5) P4 was purchased from Guangzhou Genio Biotechnology Co., Ltd. Glucose-based peritoneal dialysis solutions (PDS) included 1.5%, 2.5% and 4.25% Dianeal, from Baxter Medical Co., Ltd. Danshenol C(HPLC purity > 98%, molecular formula: C21H20O4, molecular weight: 336.3811) was purchased from Nanjing Delge Biotechnology Co., LTD. E-cadherin, VAGF-A(BS6496), MAPK8(BS6448), MAPK14(BS3566), STAT3(BS1336), Caspase3(BS1518), P-P38(AF4001), P-STAT3(S727) (AP0248), P-STAT3 (T705)(AP0247) mouse anti-human monoclonal antibody antibodies were purchased from Santa Cruz. .. Instruments used: full-wavelength microplate meter (Thermo Varioskan LUX), constant temperature incubator (Thermo Scientific), frozen high-speed centrifuge (Eppendorf 5804R, 5418R), inverted light microscope (Leica DM1000 LED), automatic incubator (Bioworld, China), and Bio-Rad chemidoc XRS (Bio-Rad, USA).

    Western Blot:

    Article Title: mTORC1 Plays an Important Role in Skeletal Development by Controlling Preosteoblast Differentiation
    Article Snippet: Nuclear and cytoplasmic extracts were prepared using NE-PER extraction reagents according 234 to the manufactures instructions (Thermo Fisher Scientific). .. Antibodies used for Western blotting: 235 Raptor (2280), P-p70 S6K T389 (9206), p70 S6K (9202), P-S6 S240/S244 (2215), S6 (2317), P-236 STAT1 S727 (8826), STAT1 (14994), P-STAT3 S727 (9134), STAT3 (9139), Runx2 (8486); Histone 237 H3 (4499) (Cell Signalling Technologies, Beverly, MA); Osterix (ab22552; Abcam); HSP90α/β (sc-238 7947, Santa Cruz Biotechnology); β-Actin (A5441, Sigma-Aldrich). .. Serum osteocalcin levels were 239 measured using a commercial ELISA (Alfa Aesar, Ward Hill, MA) as per the manufactures 240 instructions.



    Similar Products

    anti p stat3  (Bioss)
    94
    Bioss anti p stat3
    Anti P Stat3, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/STAT3(S727)+(4G1)+Monoclonal+Antibody/10__31083_slash_jin39897-83-45-48
    Average 94 stars, based on 1 article reviews
    anti p stat3 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    p stat3 s727 49 bd biosciences 624084 152sm p akt s473 d9e standard biotools 3152005a  (fluidigm)
    92
    fluidigm p stat3 s727 49 bd biosciences 624084 152sm p akt s473 d9e standard biotools 3152005a
    P Stat3 S727 49 Bd Biosciences 624084 152sm P Akt S473 D9e Standard Biotools 3152005a, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/Anti-pAkt+%5BS473%5D+(D9E)-152Sm/pm41827061-244-99-109
    Average 92 stars, based on 1 article reviews
    p stat3 s727 49 bd biosciences 624084 152sm p akt s473 d9e standard biotools 3152005a - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier
    p stat3 s727  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc p stat3 s727
    The mutual regulatory effects of MEK-Erk1/2 and <t>Stat3</t> following treatment with PD03 and C188-9, and the role of Stat3 pathways in osteogenic differentiation. (A, B) The phosphorylation levels of Erk1/2, Stat3 (Y705), and Stat3 <t>(S727)</t> in osteoblasts were examined by western blot following treatment with thrombin and PD03 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, <t>p-Stat3(Y705)</t> to Stat3, and p-Stat3(S727) to Stat3 were measured using Image J software. (C, D) The protein levels of p-Erk1/2, p-Stat3 (Y705), and p-Stat3 (S727) in osteoblasts were analyzed by western blot after being treated with thrombin and C188–9 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were evaluated using Image J software. (E) Osteoblasts were stained for ALP and Fluo-4 AM after being induced with the osteogenic differentiation medium for 7 days. (F) An enzymatic assay quantified ALP activities in cell lysates. (G) Western blot analysis was used to examine the expression levels of Col1α1, Runx2 and OCN. (H) The relative protein levels of Col1α1/β-actin, Runx2/β-actin and OCN/β-actin were measured using ImageJ software. (I) The expression levels of Col1α1, Runx2, Osterix, OPG, and OCN were assessed by qPCR following thrombin and C188–9 treatment for 7 days. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
    P Stat3 S727, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/Phospho-Stat3+(Ser727)+Antibody/pmc13006212-148-96-99
    Average 96 stars, based on 1 article reviews
    p stat3 s727 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti p stat3 s727  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti p stat3 s727
    The mutual regulatory effects of MEK-Erk1/2 and <t>Stat3</t> following treatment with PD03 and C188-9, and the role of Stat3 pathways in osteogenic differentiation. (A, B) The phosphorylation levels of Erk1/2, Stat3 (Y705), and Stat3 <t>(S727)</t> in osteoblasts were examined by western blot following treatment with thrombin and PD03 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, <t>p-Stat3(Y705)</t> to Stat3, and p-Stat3(S727) to Stat3 were measured using Image J software. (C, D) The protein levels of p-Erk1/2, p-Stat3 (Y705), and p-Stat3 (S727) in osteoblasts were analyzed by western blot after being treated with thrombin and C188–9 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were evaluated using Image J software. (E) Osteoblasts were stained for ALP and Fluo-4 AM after being induced with the osteogenic differentiation medium for 7 days. (F) An enzymatic assay quantified ALP activities in cell lysates. (G) Western blot analysis was used to examine the expression levels of Col1α1, Runx2 and OCN. (H) The relative protein levels of Col1α1/β-actin, Runx2/β-actin and OCN/β-actin were measured using ImageJ software. (I) The expression levels of Col1α1, Runx2, Osterix, OPG, and OCN were assessed by qPCR following thrombin and C188–9 treatment for 7 days. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
    Anti P Stat3 S727, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/pm41405398-444-41-43
    Average 86 stars, based on 1 article reviews
    anti p stat3 s727 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    p stat3 s727 9134t  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology p stat3 s727 9134t
    The mutual regulatory effects of MEK-Erk1/2 and <t>Stat3</t> following treatment with PD03 and C188-9, and the role of Stat3 pathways in osteogenic differentiation. (A, B) The phosphorylation levels of Erk1/2, Stat3 (Y705), and Stat3 <t>(S727)</t> in osteoblasts were examined by western blot following treatment with thrombin and PD03 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, <t>p-Stat3(Y705)</t> to Stat3, and p-Stat3(S727) to Stat3 were measured using Image J software. (C, D) The protein levels of p-Erk1/2, p-Stat3 (Y705), and p-Stat3 (S727) in osteoblasts were analyzed by western blot after being treated with thrombin and C188–9 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were evaluated using Image J software. (E) Osteoblasts were stained for ALP and Fluo-4 AM after being induced with the osteogenic differentiation medium for 7 days. (F) An enzymatic assay quantified ALP activities in cell lysates. (G) Western blot analysis was used to examine the expression levels of Col1α1, Runx2 and OCN. (H) The relative protein levels of Col1α1/β-actin, Runx2/β-actin and OCN/β-actin were measured using ImageJ software. (I) The expression levels of Col1α1, Runx2, Osterix, OPG, and OCN were assessed by qPCR following thrombin and C188–9 treatment for 7 days. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
    P Stat3 S727 9134t, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/Stat3/bio_rxiv__2025__11__17__688926-132-42-55
    Average 96 stars, based on 1 article reviews
    p stat3 s727 9134t - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    p-stat3-s727 antibody  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology p-stat3-s727 antibody
    The mutual regulatory effects of MEK-Erk1/2 and <t>Stat3</t> following treatment with PD03 and C188-9, and the role of Stat3 pathways in osteogenic differentiation. (A, B) The phosphorylation levels of Erk1/2, Stat3 (Y705), and Stat3 <t>(S727)</t> in osteoblasts were examined by western blot following treatment with thrombin and PD03 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, <t>p-Stat3(Y705)</t> to Stat3, and p-Stat3(S727) to Stat3 were measured using Image J software. (C, D) The protein levels of p-Erk1/2, p-Stat3 (Y705), and p-Stat3 (S727) in osteoblasts were analyzed by western blot after being treated with thrombin and C188–9 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were evaluated using Image J software. (E) Osteoblasts were stained for ALP and Fluo-4 AM after being induced with the osteogenic differentiation medium for 7 days. (F) An enzymatic assay quantified ALP activities in cell lysates. (G) Western blot analysis was used to examine the expression levels of Col1α1, Runx2 and OCN. (H) The relative protein levels of Col1α1/β-actin, Runx2/β-actin and OCN/β-actin were measured using ImageJ software. (I) The expression levels of Col1α1, Runx2, Osterix, OPG, and OCN were assessed by qPCR following thrombin and C188–9 treatment for 7 days. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
    P Stat3 S727 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/stat3+antibody/pmc10535698__ijbsv19p4411s1-15-55-57
    Average 90 stars, based on 1 article reviews
    p-stat3-s727 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    anti human mouse p stat3 s727  (R&D Systems)
    90
    R&D Systems anti human mouse p stat3 s727
    The mutual regulatory effects of MEK-Erk1/2 and <t>Stat3</t> following treatment with PD03 and C188-9, and the role of Stat3 pathways in osteogenic differentiation. (A, B) The phosphorylation levels of Erk1/2, Stat3 (Y705), and Stat3 <t>(S727)</t> in osteoblasts were examined by western blot following treatment with thrombin and PD03 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, <t>p-Stat3(Y705)</t> to Stat3, and p-Stat3(S727) to Stat3 were measured using Image J software. (C, D) The protein levels of p-Erk1/2, p-Stat3 (Y705), and p-Stat3 (S727) in osteoblasts were analyzed by western blot after being treated with thrombin and C188–9 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were evaluated using Image J software. (E) Osteoblasts were stained for ALP and Fluo-4 AM after being induced with the osteogenic differentiation medium for 7 days. (F) An enzymatic assay quantified ALP activities in cell lysates. (G) Western blot analysis was used to examine the expression levels of Col1α1, Runx2 and OCN. (H) The relative protein levels of Col1α1/β-actin, Runx2/β-actin and OCN/β-actin were measured using ImageJ software. (I) The expression levels of Col1α1, Runx2, Osterix, OPG, and OCN were assessed by qPCR following thrombin and C188–9 treatment for 7 days. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
    Anti Human Mouse P Stat3 S727, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3+s727/Human+Phospho-STAT3+(S727)+Antibody/pmc12277254-109-24-28
    Average 90 stars, based on 1 article reviews
    anti human mouse p stat3 s727 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The mutual regulatory effects of MEK-Erk1/2 and Stat3 following treatment with PD03 and C188-9, and the role of Stat3 pathways in osteogenic differentiation. (A, B) The phosphorylation levels of Erk1/2, Stat3 (Y705), and Stat3 (S727) in osteoblasts were examined by western blot following treatment with thrombin and PD03 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were measured using Image J software. (C, D) The protein levels of p-Erk1/2, p-Stat3 (Y705), and p-Stat3 (S727) in osteoblasts were analyzed by western blot after being treated with thrombin and C188–9 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were evaluated using Image J software. (E) Osteoblasts were stained for ALP and Fluo-4 AM after being induced with the osteogenic differentiation medium for 7 days. (F) An enzymatic assay quantified ALP activities in cell lysates. (G) Western blot analysis was used to examine the expression levels of Col1α1, Runx2 and OCN. (H) The relative protein levels of Col1α1/β-actin, Runx2/β-actin and OCN/β-actin were measured using ImageJ software. (I) The expression levels of Col1α1, Runx2, Osterix, OPG, and OCN were assessed by qPCR following thrombin and C188–9 treatment for 7 days. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.

    Journal: Frontiers in Immunology

    Article Title: PD0325901 alleviates thrombin-inhibited osteogenic differentiation through an IL-1β-activated feedback loop between MEK-Erk1/2 and NF-κB signal pathways: insights from bioinformatics and experimental verification

    doi: 10.3389/fimmu.2026.1730337

    Figure Lengend Snippet: The mutual regulatory effects of MEK-Erk1/2 and Stat3 following treatment with PD03 and C188-9, and the role of Stat3 pathways in osteogenic differentiation. (A, B) The phosphorylation levels of Erk1/2, Stat3 (Y705), and Stat3 (S727) in osteoblasts were examined by western blot following treatment with thrombin and PD03 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were measured using Image J software. (C, D) The protein levels of p-Erk1/2, p-Stat3 (Y705), and p-Stat3 (S727) in osteoblasts were analyzed by western blot after being treated with thrombin and C188–9 at different time points, and the relative protein levels of p-Erk1/2 to Erk1/2, p-Stat3(Y705) to Stat3, and p-Stat3(S727) to Stat3 were evaluated using Image J software. (E) Osteoblasts were stained for ALP and Fluo-4 AM after being induced with the osteogenic differentiation medium for 7 days. (F) An enzymatic assay quantified ALP activities in cell lysates. (G) Western blot analysis was used to examine the expression levels of Col1α1, Runx2 and OCN. (H) The relative protein levels of Col1α1/β-actin, Runx2/β-actin and OCN/β-actin were measured using ImageJ software. (I) The expression levels of Col1α1, Runx2, Osterix, OPG, and OCN were assessed by qPCR following thrombin and C188–9 treatment for 7 days. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.

    Article Snippet: The following antibodies were used in this study: β-actin (AF5001, Beyotime, China), Col1α1 (ab270993, Abcam, UK), Runx2 (AF2593, Beyotime, China), OCN (AF6297, Beyotime, China), Igf1 (AF7179, Beyotime, China), Wnt5a (29793-1-AP, Proteintech, USA), Tgfb3 (AF8142, Beyotime, China), Spp1 (A5427, Bimake, USA), COX-2 (F0327, Bimake, USA), Matrix Metalloproteinase-9 (MMP-9, AF5234, Beyotime, China), Minichromosome Maintenance Complex Component 2 (MCM2, A5172, Bimake, USA), Proliferating Cell Nuclear Antigen (PCNA, SC-25280, Santa, USA), PAR-1 (AF6837, Beyotime, China), IL-1RA (AF7218, Beyotime, China), p65 (8242S, CST, USA), p-p65 (3033T, CST, USA), Erk1/2 (4695T, CST, USA), p-Erk1/2 (4370S, CST, USA), p-Stat3 (Y705) (9145S, CST, USA), p-Stat3 (S727) (9134S, CST, USA), and Stat3 (4904T, CST, USA).

    Techniques: Phospho-proteomics, Western Blot, Software, Staining, Enzymatic Assay, Expressing